Introduction to Sunresin’s Core-Shell Multimodal Chromatography Media

Application of Seplife Multimodal Chromatography Resins in Downstream Purification of Commercial Production of Virus Vaccines--- Introduction to Sunresin’s Core-Shell Multimodal Chromatography Media

The Core-Shell multi-mode chromatography medium is a new multi-mode agarose chromatography medium independently developed by Sunresin Technology, used for purification and polishing of biological macromolecules such as viruses.

The chromatographic medium uses a new type of shell microsphere technology and octylamine ligand, and has dual functions of gel filtration and adsorption separation.

The core functional area is bound with a strong adsorption ligand, octylamine, which binds molecules under a wide range of pH and ionic strength by electrostatic force and hydrophobic force. At the same time, the outer layer of the core functional area has a shell layer of about 5um, with 700 KDa / 400 KDa gaps on the shell layer that are connected to the core functional area.

Sunresin’s new Core-Shell Multimodal chromatography can efficiently capture contaminants while allowing target molecules to flow through.

The Seplife ® Suncore 700 / 400  chromatography resins  have the characteristics of high flow rate, low back pressure, high throughput, good chemical stability and mechanical properties, which facilitate scale-up, shorten production time and improve production efficiency.

When the clarified solution flows through the column bed, biomolecules larger than 700 KDa/400 KDa are excluded from the “shell” and flow through the outer water volume, while biomolecules smaller than 700 KDa/400 KDa enter the core functional area through the gaps in the shell layer and are combined in the “core”, thus achieving the separation of ultra-large biomolecules and other biomolecules even in the state of higher flow rate and larger sample load.

(Sunresin’s Seplife Suncore 400 multimodal resins purifying the virus vaccine in a 600mm chromatographic column.)

Core-Shell Multimodal Chromatography Media Technical Paremeters